Bismark: WGBS 전처리

Bismark: WGBS 전처리 #

#2025-04-21

1. Build Index #

$ bowtie2-build Homo_sapiens.GRCh38.dna.toplevel.fa GRCh38 -p 40

2. Bam Sorting & Indexing #

$ samtools sort KEB01_1_bismark_bt2_pe.bam -o KEB01_1_bismark_bt2_pe.sorted.bam

$ samtools index KEB01_1_bismark_bt2_pe.sorted.bam

3. Methylation Extraction #

$ bismark_methylation_extractor --gzip --bedGraph --buffer_size 10G --cytosine_report --genome_folder /data3/PUBLIC_DATA/ref_genomes/homo_sapiens/GRCh37_hg19/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta KEB01_1_bismark_bt2_pe.sorted.bam